KIM, Woon Soo* & CHOI, Yae Chin**(University of Ill., U.S.A.* & Chunchon Agricultural Coll.**) Studies on the viruses of radish mosaic. Kor. Jour. Bot. VI (2):9-21, 1963.
A mosaic diseased radish collected from the suburb of Seoul, in November, 1961 was used for studing the host range, physical properties, purification, insect transmission, and electron microscopy.
A Japanese strain of raddish mosraic (RPV) was also used with Korean strain of radish mosaic (KRMV) for a comparative study.
The two viruses, KRMA and RPV, were identified by the difference in host range, insect transmission and electron microscopy. The KRMA was severely infective on tobacco and Nicotiana glutinosa, while on Gomphrena globosa was immune to the virus. RPV produces necrotic local lesions on Comphrena globosa but did not infect tobacco and N. glutinosa. Among varieties of radish, Seoul, Akamaroo, Akanagea, Koong-Joong showed more severe symptoms than Simoo, Minong, Paek-soo, which appeared to be fainly resistant. In a number of tests, it was found that the virus KRMA retained its infectivity until to a dilution of 1:2,000, heating at 58˚C for 10 minutes, and aging in vitro for 7 days at room temperature. The RPV was not inactivated until it was diluted to 1:2,000, heated to 56。C, and aged for 6days.
The KRMV was readily transmitted by the aphid (Myzus persicae Sulz). The virus RPV was not transmitted by the aphid in a number of tests. Partialy purified viruses using ammonium acetate buffer, salting-out by ammonium sulfate and centrifugation of high and low speed were highly infective. Electron micrographs showed that the KRMV paticles are of spherical particles whereas the RPV particles are rod-shaped.
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